grant
Use of Bacteriophages to Prevent, Diagnose, and Treat Diseases
Organization DIVISION OF BASIC SCIENCES - NCILocation UNITED STATES
NIHUS FederalResearch GrantFY2025AntigensAssayBacterial InfectionsBacteriophage lambdaBacteriophagesBioassayBiological AssayBiotechBiotechnologyBlood SerumCancersColiphage lambdaCommunicable DiseasesDiagnosisDiseaseDisorderDrug resistanceEGF ReceptorEGFREGFRvIIIELISAERBB ProteinEnterobacteria phage lambdaEnzyme-Linked Immunosorbent AssayEpidermal Growth Factor ReceptorEpidermal Growth Factor Receptor KinaseEpidermal Growth Factor Receptor Protein-Tyrosine KinaseEpidermal Growth Factor-Urogastrone ReceptorsExternal DomainExtracellular DomainGeneticGlioblastomaGrade IV Astrocytic NeoplasmGrade IV Astrocytic TumorGrade IV AstrocytomaHER1HeadImmunoblottingInfectious DiseasesInfectious DisorderMalignant NeoplasmsMalignant TumorMiceMice MammalsMurineMusPeptidesPhage DisplayPhage lambdaPhagesProteinsPublishingSerumSpecificitySystemTGF-alpha ReceptorTechnologyTestingTransforming Growth Factor alpha ReceptorUrogastrone ReceptorVaccinesVirionVirus ParticleWestern BlottingWestern Immunoblottingbacteria infectionbacterial diseasebacterial virusc-erbB-1c-erbB-1 Proteindevelop a vaccinedevelop vaccinesdevelopment of a vaccinedevelopmental geneticsdrug resistantenzyme linked immunoassayepidermal growth factor receptor VIIIerbB-1erbB-1 Proto-Oncogene ProteinerbBlexperimentexperimental researchexperimental studyexperimentsglioblastoma multiformehuman diseaseimmunogenmalignancymouse modelmurine modelneoplasm/cancernew technologynovel technologiespreventpreventingprotein blottingproto-oncogene protein c-erbB-1resistance to Drugresistant to Drugspongioblastoma multiformetumorvaccine development
Description preview
The extracellular domain of EGFRvIII was displayed on Lambda virion by genetic fusion of the EGFRvII peptide to Lambda head protein D. Upon administration of the this Lambda virion into experimental mice, it generated mice serum which specifically interct with EGFRvIII protein but not with wildtype EGFR . The serum The specificity tests were…
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