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Structure-function mapping of large cargo transport through the nuclear pore complex

Organization ROCKEFELLER UNIVERSITYLocation NEW YORK, UNITED STATESPosted 1 Feb 2024Deadline 31 Jan 2026 ⚠️
NIHUS FederalResearch GrantFY202520S Catalytic Proteasome20S Core Proteasome20S Proteasome20S ProteosomeActive Biologic TransportActive Biological TransportActive TransportAddressAdoptedAffectAffinityAminoacetic AcidAssayAtomic EnergyBaker's YeastBehaviorBinding SitesBioassayBiological AssayBiological FunctionBiological ProcessBrewer's YeastBudding YeastCancersCapsidCell BodyCell FunctionCell NucleusCell Nucleus Active TransportCell PhysiologyCell ProcessCellsCellular FunctionCellular PhysiologyCellular ProcessCombining SiteCommunicable DiseasesCouplingCryo-electron MicroscopyCryoelectron MicroscopyCytoplasmDNADNA TherapyDataDefectDeoxyribonucleic AcidDependenceDevelopmentDiseaseDisorderDrug DeliveryDrug Delivery SystemsDrug TargetingDrugsElectron CryomicroscopyElementsEndomycetalesEukaryotic CellFluorescence Light MicroscopyFluorescence MicroscopyGene ExpressionGene Transfer ClinicalGenetic InterventionGenomeGlycineGoalsHBVHIV InfectionsHIV-1HIV-IHIV1HTLV-III InfectionsHTLV-III-LAV InfectionsHepatitis B VirusHumanHuman Immunodeficiency Virus Type 1Human T-Lymphotropic Virus Type III InfectionsHuman immunodeficiency virus 1In SituInfectionInfectious DiseasesInfectious DisorderInterphaseInvestigatorsLocationMacropainMacroxyproteinaseMalignant NeoplasmsMalignant TumorMapsMass Photometry/Spectrum AnalysisMass SpectrometryMass SpectroscopyMass SpectrumMass Spectrum AnalysesMass Spectrum AnalysisMediatingMedicationMembraneModelingModern ManMolecularMolecular TransportMulticatalytic ProteinaseNPCNervous System DiseasesNervous System DisorderNeurologic DisordersNeurological DisordersNon-Polyadenylated RNANuclearNuclear EnergyNuclear EnvelopeNuclear MembraneNuclear Pore ComplexNuclear Pore Complex ProteinsNuclear TransportNucleocytoplasmic ShuttlingNucleoporin GeneNucleoporinsNucleusNup ProteinOncogenicPeptide DomainPharmaceutical PreparationsPhenotypePhenylalanineProbabilityProcessProsomeProteasomeProteasome Endopeptidase ComplexProtein DomainsProteinsProteosomeRNARNA Gene ProductsRadialRadiusReactive SiteResearchResearch PersonnelResearchersResolutionRibonucleic AcidRibosomesRoleRunningS cerevisiaeS. cerevisiaeSaccharomyces cerevisiaeSaccharomycetalesSideSiteStructureSubcellular ProcessSystemTertiary Protein StructureTherapeuticTransmissionTransport ProcessUphill TransportViralVisualizationWidthYeastscryo-EMcryoEMcryogenic electron microscopydensitydevelopmentaldevelopmental diseasedevelopmental disorderdrug/agentfitnessgene repair therapygene therapygene-based therapygenetic therapygenomic therapymRNPmalignancymembrane structuremessenger ribonucleoproteinmulticatalytic endopeptidase complexmutantnano particlenano-sized particlenanocarriernanoparticlenanosized particlenanovesselneoplasm/cancerneurological diseasenon-Nativenonnativenucleocytoplasmic transportpreservationresolutionsscaffoldscaffoldingsmall moleculesocial roletransmission process

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PROJECT SUMMARY/ABSTRACT
The eukaryotic cell has evolved to compartmentalize DNA within the nucleus, surrounded by the barrier of the

nuclear envelope (NE), providing protection to the DNA and a means to control information flow to and from the

genome. This flow, namely the passage of small molecules, RNAs, and proteins across the NE, is mediated…

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Structure-function mapping of large cargo transport through the nuclear pore complex — ROCKEFELLER UNIVERSITY | UNITED S | Dev Procure