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Project I - Transcriptomic Analysis of Structural Birth Defects in Mouse Developmental Mutants

Organization SEATTLE CHILDREN'S HOSPITALLocation SEATTLE, UNITED STATESPosted 11 Jan 2021Deadline 31 Dec 2025 โš ๏ธ
NIHUS FederalResearch GrantFY2025Anatomic SitesAnatomic structuresAnatomyBiologicalBirth DefectsCell BodyCell Communication and SignalingCell SignalingCellsComputational toolkitComputer AnalysisCongenital AbnormalityCongenital Anatomical AbnormalityCongenital DefectsCongenital DeformityCongenital MalformationDNA mutationDataData SetDevelopmentEmbryoEmbryo DevelopmentEmbryogenesisEmbryonicEmbryonic DevelopmentFrequenciesGene ExpressionGene Expression MonitoringGene Expression Pattern AnalysisGene Expression ProfilingGenerationsGenesGenetic ChangeGenetic defectGenetic mutationGestationGroupingHeterozygoteHumanIntracellular Communication and SignalingLethal GenesMethodsMiceMice MammalsModern ManMurineMusMutateMutationNon-Polyadenylated RNAOrganogenesisPathway interactionsPhenotypePlayPregnancyProteinsRNARNA Gene ProductsRNA SeqRNA SequencesRNA sequencingRNAseqRibonucleic AcidRoleSeverity of illnessSignal PathwaySignal TransductionSignal Transduction SystemsSignalingStructural Birth DefectStructural Congenital AnomaliesTechniquesTechnologyTestingTranscript Expression AnalysesTranscript Expression Analysisanalyze gene expressionbio-informatics toolbioinformatics toolbiologicbiological signal transductioncell typecohortcombinatorialcomparativecomputational analysescomputational analysiscomputational toolboxcomputational toolscomputational toolsetcomputer analysescomputerized toolscongenital structural malformationdevelopmentaldiscover genesdisease severityexome sequencingexome-seqgene discoverygene expression analysisgene expression assaygenome mutationgroupingsheterozygosityhuman diseaseindexinginsightmethod developmentmutantnovelpathwayscRNA sequencingscRNA-seqsingle cell RNA-seqsingle cell RNAseqsingle cell analysissingle cell expression profilingsingle cell transcriptomic profilingsingle-cell RNA sequencingsocial rolestatisticstranscriptional profilingtranscriptome sequencingtranscriptomic sequencingtranscriptomics

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PROJECT SUMMARY
In this project we plan to apply a powerful and scaleable technique of combinatorial indexing to characterize

gene expression at the single-cell level in mice carrying mutations that are either known to or likely to result in

structural birth defects. We propose to assess whether single-cell expression can be utilized as aโ€ฆ

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