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Organization UNIVERSITY OF MISSOURI-COLUMBIALocation COLUMBIA, UNITED STATESPosted 1 Jun 2001Deadline 30 Apr 2026 ⚠️
NIHUS FederalResearch GrantFY2025AccelerationAffectAmino AcidsApplied ResearchApplied ScienceArchivesAreaCRISPR approachCRISPR based approachCRISPR methodCRISPR methodologyCRISPR techniqueCRISPR technologyCRISPR toolsCRISPR-CAS-9CRISPR-based methodCRISPR-based techniqueCRISPR-based technologyCRISPR-based toolCRISPR/CAS approachCRISPR/Cas methodCRISPR/Cas technologyCRISPR/Cas9CRISPR/Cas9 technologyCapacitation of SpermatozoaCas nuclease technologyClustered Regularly Interspaced Short Palindromic Repeats approachClustered Regularly Interspaced Short Palindromic Repeats methodClustered Regularly Interspaced Short Palindromic Repeats methodologyClustered Regularly Interspaced Short Palindromic Repeats techniqueClustered Regularly Interspaced Short Palindromic Repeats technologyCommon Rat StrainsCryofixationCryopreservationDataDevelopmentES cellEmbryo DevelopmentEmbryogenesisEmbryonic DevelopmentEnsureExtinctionFertilizationFertilization in VitroFreezingFundingFutureGene ModifiedGenerationsGenesGenetics-MutagenesisGermGoalsICSIIn VitroInbred StrainIncubatedInjectionsIntracytoplasmic Sperm InjectionsInvestigatorsLaboratory ResearchLettersMeasuresMethodsMiceMice MammalsModelingMotilityMurineMusMutagenesisMutagenesis Molecular BiologyOocytesOutcomeOutcome MeasureOvocytesProtocolProtocols documentationPublishingQualifyingRatRat StrainsRats MammalsRattusRenaissanceReportingReproductive BiologyResearchResearch PersonnelResearch ResourcesResearchersResourcesSpermSperm CapacitationSperm MotilitySpermatogenesisSpermatozoaSupplementationTAL effector nucleaseTAL endonucleaseTALE nucleaseTALEN technologyTALENsTechniquesTechnologyTest-Tube FertilizationTestingTransgenic Organismsaminoacidanimationbase editingcold preservationcold storagecostcost effectivecryobiologydevelopmentalembryo derived stem cellembryonal stem cellsembryonic progenitorembryonic stem cellexperienceexperimentexperimental researchexperimental studyexperimentsfertilizationsgene manipulationgene modificationgenetic manipulationgenetically manipulategenetically modifiedgenetically perturbgenome editinggenomic editingimprovedknock-out animalknockout animalmeasurable outcomenovelnucleaseoocyte maturationoperationoperationsoutcome measurementpreservationreproductivesemen cryopreservationsperm cellsperm cell cryopreservationsperm cryopreservationsperm freezingsperm mobilityspermatozoa cryopreservationstem cell of embryonic originsuccesstranscription activator-like effector nucleasestransgeniczoosperm

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Abstract (Applied Research)
The generation of novel genetically modified rat models has greatly accelerated with the availability of rat
embryonic stem cells and the advent of nuclease-based genome-editing technologies such as ZFNs, TALENs,
and CRISPR/Cas9. There is a need for reliable and cost-effective methods of cryopreservation to archive…

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