Multi-omics profiling of individual exosomes for origin-tracing, biomarker discovery, and biological function characterization

PROJECT SUMMARY / ABSTRACT
Extracellular vesicles (EVs) exhibit high heterogeneity in biofluids, a feature that traditional bulk-level analysis
approaches fail to capture in terms of individual variations. While various techniques exist for single-EV analysis,
the majority focus primarily on surface proteins. Transcriptional analysis at the level of individual EVs, however,
remains largely unexplored. To bridge this gap, we propose the development of a platform for multimodal digital
profiling of individual EVs, leveraging next-generation sequencing and an optimized system for multiplex library
preparation. This proposed platform will serve as a unique tool for high-throughput absolute quantification of
single-EV genes and proteins. It aims to offer high-sensitivity, multi-dimensional biological insights, thereby
potentially accelerating the advancement of EV-based diagnostics and targeted therapies.

Grant Number: 3R44GM145015-03S1
NIH Institute/Center: NIH
Principal Investigator: Yuchao Chen