Multi-omics profiling of individual exosomes for origin-tracing, biomarker discovery, and biological function characterization

PROJECT SUMMARY / ABSTRACT
Extracellular vesicles (EVs) exhibit high heterogeneity in biofluids, a feature that traditional bulk-level analysis
approaches fail to capture in terms of individual variations. While numerous techniques exist for single-EV
analysis, the majority focus primarily on profiling surface proteins. Transcriptional analysis at the level of
individual EVs, however, remains largely unexplored. To bridge this gap, we propose the development of a
technology for multimodal profiling of individual EVs, leveraging next-generation sequencing and an optimized
method for multiplex library preparation. This proposed platform will serve as a unique tool for high-throughput,
integrative profiling of single-EV gene expression and surface proteins. It aims to offer high-sensitivity, multi-
dimensional biological insights, thereby potentially accelerating the advancement of EV-based diagnostics and
targeted therapies.

Grant Number: 5R44GM145015-03
NIH Institute/Center: NIH
Principal Investigator: Yuchao Chen