Molecular mechanisms underlying HIV & Cocaine-mediated microglial activation: Targeting NLRP3 inflammasome

In the current era of combination antiretroviral therapy (cART), there is increased prevalence of HIV-associated
neurocognitive disorders (HAND) with about 30-50% of HIV-infected individuals afflicted with varying degrees of
neurocognitive impairments, substantially affecting their quality of life. It is well-recognized that despite cART,
there is unabated persistence & presence of early HIV protein(s) such as the cytotoxic transactivator of
transcription (TAT), that contributes to neuroinflammation. Adding fuel to the mix is the comorbidity of drug abuse
in HIV-infected individuals, which further exacerbates microglial activation & thus symptomatology of HAND.
Until recently, CNS was considered as an immune-privileged site, however, a recent paradigm shift in our
understanding has revealed the role of cytosolic, multiprotein complexes, belonging to the nucleotide-binding
domain leucine-rich repeat containing (NLR) protein superfamily, termed as “inflammasomes,” in various
neuroinflammatory diseases. Assembly of these multiprotein complexes activates the proinflammatory caspases
(specifically caspase 1), leading, in turn, to the cleavage & release of IL1β & 1L18, consequently resulting in a
potent inflammatory response. Among the various NLRs, NLRC5 & NLRP3 are highly expressed in microglia & differentially regulate inflammatory responses. In our efforts to understand the combinatorial effects of HIV Tat & cocaine on Mg activation, we have made several exciting preliminary observations: 1) Exposure of rodent
primary Mg to both HIV Tat & cocaine demonstrated significantly downregulated expression of NLRC5, with a
concomitant upregulation of NLRP3 and, exacerbated Mg activation compared to cells exposed to either agent
alone; 2) Rodent Mg exposed to HIV TAT demonstrated time-dependent upregulation of microRNA (miR)-34a,
leading, in turn, to downregulation of its target - NLRC5; 3) Cocaine-mediated activation of Mg involved induction
of reactive oxygen species (ROS), that was accompanied with defective mitophagy & subsequent
oligomerization of NLRP3 inflammasome complex, leading to induced expression of mature (m)IL1β & IL18; 4)
NLRP3 inhibitor - MCC950 mitigated cocaine-mediated activation of Mg. Based on these observations, we
hypothesize that the co-operative effects of HIV TAT & cocaine on Mg activation involve two processes: a)
downregulated expression of NLRC5 resulting in activation of NFκB (signal 1), leading, in turn, to transcriptional
upregulation of NLRP3, pro IL1β & IL18, & b) induction of ROS-mediated defective mitophagy (signal 2), which,
in turn, induces activation of NLRP3 inflammasome, resulting in increased cleavage & secretion of mIL1β & IL18.
The hypothesis will be tested in two SA: 1) Explore the molecular mechanism(s) underlying HIV & cocaine-
mediated induction of the NLRP3 inflammasome leading to Mg activation and 2) To validate in vivo the role of
NLRC5/NFκB & ROS/defective mitophagy/NLRP3 axes underlying HIV Tat & cocaine-mediated activation of Mg.
Understanding the mechanisms responsible for microglial activation induced by HIV & cocaine will set the stage
for the future development of novel therapeutics aimed at dampening the neuroinflammatory responses.

Grant Number: 5R01DA050545-05
NIH Institute/Center: NIH
Principal Investigator: Shilpa Buch