grant
Laboratory Studies of Human Respiratory Syncytial Virus and Other Pneumoviruses
Organization NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASESLocation UNITED STATES
NIHUS FederalResearch GrantFY2025AgeAmino AcidsAnimal ModelAnimal Models and Related StudiesAssayAttenuatedAttenuated VaccinesBasic ResearchBasic ScienceBioassayBiological AssayCRADACardiopulmonaryCharacteristicsChildhoodClinical EvaluationClinical ResearchClinical StudyClinical TestingCodeCoding SystemCodonCodon NucleotidesComputational algorithmCooperative Research and Development AgreementCytoplasmDNA Molecular BiologyDNA mutationDevelopmentElderlyEvaluationFamily memberGene AlterationGene MutationGene ProteinsGenesGeneticGenetic ChangeGenetic defectGenetic mutationGenomeGlycoproteinsGoalsHumanHuman MetapneumovirusHydrophobicityImmunobiologyImmunophysiologyIndividualLaboratory StudyLicensingLive-attenuated VaccineM proteinMarketingMessenger RNAModern ManMolecular BiologyMolecular GeneticsMolecular VirologyMorbidityMorbidity - disease rateMurine pneumonia virusMutationNon-Polyadenylated RNANon-structural ProteinNonstructural ProteinNucleoproteinsORFsOpen Reading FramesOrthopneumovirusPhasePhosphoproteinsPlayPneumonia Virus of MicePneumoviridaePneumovirinaePneumovirusPoint MutationPolymerasePopulationProtein Coding RegionProtein Gene ProductsProteinsRNARNA Gene ProductsRNA VirusesRSV VaccinesReagentRefractoryReportingRespiratory Syncytial Virus VaccinesRespiratory Tract DiseasesRespiratory syncytial virusRibonucleic AcidRodentRodentiaRodents MammalsRoleRouteSerology testSystemTemperatureVaccine Clinical TrialVaccinesVariantVariationViralViral Gene ProductsViral Gene ProteinsViral ProteinsVirusadvanced ageagesaminoacidattenuateattenuatesattenuationclinical candidateclinical developmentclinical testcomputer algorithmdesigndesigningdevelop a vaccinedevelop vaccinesdevelopment of a vaccinedevelopmentalexperimentexperimental researchexperimental studyexperimentsgene defectgene synthesisgenome mutationgenome scalegenome-widegenomewidegeriatricglycoprotein Ghypoimmunityimmune deficiencyimmunodeficiencyimprovedlead candidatelive vaccinelive vaccinesmRNAmembermodel of animalmortalitymultiple myeloma M Proteinmutantmutant allelepathogenpediatricpre-clinical developmentpre-clinical studypreclinical developmentpreclinical studyprogramsresearch clinical testingreverse geneticssenior citizenserology assaysocial rolevaccine candidatevaccine developmentviral fitnessvirus protein
Description preview
We previously performed codon-pair deoptimization (CPD) of various open reading frames (ORFs) of RSV. This is done by rearranging codons of RSV open reading frames using computer algorithms and de novo gene synthesis to increase the content of normally underrepresented codon-pairs without changing amino acid coding or overall codon usage. CPD…
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