grant
Identification of genomic regulatory elements in pancreas cells
Organization DIVISION OF BASIC SCIENCES - NCILocation UNITED STATES
NIHUS FederalResearch GrantFY20253-D3-Dimensional3DAcinar CellAciner CellsAssayBioassayBiological AssayCRISPR interferenceCRISPR-dCas9-mediated repressionCRISPR/dCas9 interferenceCRISPR/dCas9-mediated transcriptional inhibitionCRISPRiCancersCell BodyCell LineageCellsChromatinClustered Regularly Interspaced Short Palindromic Repeats interferenceCoupledDNADNA SequenceDataData SetDeoxyribonucleic AcidDiabetes MellitusDiathesisDisease susceptibilityDuctal CellDuctal Epithelial CellEndocrineEnhancersGene Action RegulationGene ExpressionGene Expression RegulationGene RegulationGene Regulation ProcessGene variantGenesGeneticGenetic AnnotationGenomicsGoalsGraphHumanImageInvestigationLinkMachine LearningMalignant NeoplasmsMalignant Pancreatic NeoplasmMalignant TumorMalignant neoplasm of pancreasMapsMeasuresModelingModern ManNon-Polyadenylated RNAPancreasPancreas CancerPancreaticPancreatic CancerPancreatic DiseasesPancreatic DisorderPredisposition geneRNARNA Gene ProductsRegulatory ElementResearchRibonucleic AcidSusceptibility GeneTreesValidationVariantVariationWorkallelic variantcancer riskcell typediabetesdisease riskdisorder riskgenetic variantgenome wide analysisgenome wide studiesgenome-wide analysisgenome-wide identificationgenomic variantimaginginterestliability to diseasemachine based learningmachine learned algorithmmachine learning algorithmmachine learning based algorithmmalignancyneoplasm/cancernovelpancreas disorderpancreatic malignancypredisposing geneprogramspromoterpromotorrepressing CRISPR-dCas9 systemsusceptibility allelesusceptibility locussusceptibility variantthree dimensionalvalidations
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Description preview
Using 3D chromatin assays, we generated an extensive enhancer interaction dataset for the human pancreas, spanning more than 20 donors and five major cell types, including both the exocrine and endocrine compartments. We employed a network approach to parse chromatin interactions into enhancer-promoter tree models, facilitating quantitative,…
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