grant

Humanized Mouse Core

Organization OREGON HEALTH & SCIENCE UNIVERSITYLocation PORTLAND, UNITED STATESPosted 15 Aug 2017Deadline 31 Jul 2027
NIHUS FederalResearch GrantFY2025AMD-3100AMD3100Animal ModelAnimal Models and Related StudiesBlood Precursor CellBody TissuesCD34CD34 geneCMVCSF3CSF3 geneCell BodyCell Communication and SignalingCell SignalingCellsChemicalsClinicalCollaborationsCord BloodCytomegalovirusDNA TherapyEngraftmentExhibitsFetal LiverFetal Tissue ResearchG-CSFGCSFGene Transfer ClinicalGeneralized GrowthGenerationsGenesGenetic InterventionGoalsGrowthHCMVHPCA1HealthHematopoietic Progenitor CellsHematopoietic Stem Cell MobilizationHematopoietic progenitor MobilizationHematopoietic progenitor cell MobilizationHematopoietic stem cellsHumanHuman BiologyIn VitroInfectionInfrastructureIntracellular Communication and SignalingInvestigationIsoformsKnowledgeLatent virus infection phaseMGC45931MacrophageMiceMice MammalsModelingModern ManMolecularMurineMusOregonPlerixaforProgenitor Cell EngraftmentProgenitor CellsProgenitor cell mobilizationProtein IsoformsPublishingR-Series Research ProjectsR01 MechanismR01 ProgramReportingResearchResearch GrantsResearch Project GrantsResearch ProjectsRoleSalivary Gland VirusesScienceSignal PathwaySignal TransductionSignal Transduction SystemsSignalingSourceStandardizationStem Cell MobilizationStem Cell traffickingSupervisionSupporting CellSystemTestingTissue GrowthTissuesUmbilical Cord BloodUniversitiesVAC-TXVaccine TherapyViralViral LatencyVirusVirus LatencyVirus ReplicationWorkbiological signal transductionblood cell progenitorblood progenitorblood stem cellblood-forming stem cellcell typecytomegalovirus groupdesigndesigningexperimentexperimental researchexperimental studyexperimentsfetal cord bloodgene repair therapygene therapygene-based therapygenetic therapygenomic therapyhematopoietic progenitorhematopoietic stem and progenitor cell mobilizationhematopoietic stem progenitor cellhemopoietic progenitorhemopoietic stem cellhuman fetal tissuehuman fetus tissuehuman tissuehumanized micehumanized mousehypoimmunityimmune deficiencyimmunodeficiencyin vivoinhibitorlatency/reactivationlatent viral infectionlatent virus infectionmodel of animalmouse modelmurine modelmutantontogenyprogenitor mobilizationprogramsreactivation from latencyshRNAshort hairpin RNAsmall hairpin RNAsocial rolestem cell engraftmentstem cellstherapeutic vaccinationviral multiplicationviral replicationvirus multiplication
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Full Description

SUMMARY - HUMANIZED MOUSE CORE (CORE A)
The huNSG mouse model nicely recapitulates aspects of human biology that are not supported in other

systems. This humanized mouse model also offers the only small animal model capable of supporting HCMV

infection, latency, and reactivation. This PPG requires our highly standardized huNSG model in order to

effectively characterize unique conditions and quantitatively differentiate viral growth and dissemination of

different viral mutants and treatments. Core A will provide the infrastructure and knowledge required for

completion of the proposed research projects involving huNSG mice and standardize analysis between all

projects to allow for direct comparisons. By working directly with the Project leaders, Core A will aid in

designing and execution of the proposed experiments.

Grant Number: 5P01AI127335-09
NIH Institute/Center: NIH

Principal Investigator: Patrizia Caposio

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