HIV Virology Core

HIV Virology Core
To support the needs of the three PCHPI projects, recipients of Collaborative Development Awards, and external
collaborative projects, we will establish an HIV Virology Core (HVC). The primary missons of the HVC will be to
test hypotheses resulting from new structural information generated by the center projects and to drive studies
that are complementary to the proposed structural work, as detailed in the project descriptions. The HVC will
achieve these goals by performing functional studies of HIV-1 using a battery of assays and tools of the trade.
Three groups, led by experienced investigators, will contribute to the effort: Chris Aiken (specializing in assays
of HIV-1 replication; purification and analysis of HIV-1 cores; functional studies of HIV-1 mutants; and assays of
endogenous reverse transcription); Alan Engelman (specializing in functional studies of HIV-1 integration,
including deep sequencing analysis of integration sites; gene knock-out and recoding knock-in experiments to
anlyze effects of cell mutations on HIV-1 infection, with an emphasis on nuclear entry and integration defects;
quantitative assessment of virion morphology by transmission electron microscopy; and work with primary
human cell cultures); and Zandrea Ambrose (specializing in single-cycle infection assays; in vitro analysis of
single-particle uncoating; staging of HIV-1 infection defects; and cell-based imaging studies of HIV-1 ingress,
including intracellular trafficking, gene knockdown/knockout of trafficking proteins, and infections of primary
cells). The HVC will be collaboratively managed by Aiken and Engelman, an arrangement that has served the
PCHPI well and will ensure that the Projects are appropriately supported.

Grant Number: 5U54AI170791-04
NIH Institute/Center: NIH
Principal Investigator: Christopher Aiken