Flow Cytometry Core

Summary
The North Dakota Flow Cytometry and Cell Sorting (ND-FCCS) Core was established to provide investigators
and their laboratory staff associated with this COBRE, the North Dakota IDeA Networks of Biomedical
Research Excellence (INBRE), and the Dakota Cancer Collaborative on Translational Activity (DaCCoTA)
CTR, as well as researchers across the state of North Dakota, the following: consultation in the design of
fluorescent cell analysis or sorting experiments including fluorochrome selection and parameter calibration;
training and oversight on the instrumentation to collect flow cytometry data; flow cytometric cell sorting;
consultation on data analysis and usage of analysis software; as well as maintenance, upkeep, and when
appropriate, upgrading of the instrumentation and analysis software. During Phase 1, the Core was utilized
heavily by all of the researchers holding COBRE projects as well as most of the laboratories associated with
the larger infectious disease group, investigators in the ND INBRE at UND, NDSU, and Minot State University,
and other non-associated investigators at UND, NDSU, and the USDA Human Nutrition Center. During Phase
1 the Core was able to replace the obsolete BD LSR2 with a BD Symphony with funds from both this COBRE
and the ND INBRE, and later to add a third flow cytometer, a Sony MA900 Cell Sorter, with funds from the
DaCCoTA CTR, as well as to increase the number of licenses for FlowJo analytical software held by the Core
for use predominately by the HPI COBRE investigators. The Core will continue with the services provided
moving into Phase 2, as well as initiating full service for those investigators wanting the Core to perform the
collection of flow cytometric data on their prepared/stained cells, as well as continued fluorescent cell sorting
for the COBRE, INBRE, and CTR investigators, as well as non-associated researchers across North Dakota.
To ensure ongoing sustainability, a fee for service or instrument usage fee will be implemented during the
Phase 2 period of the COBRE for all users of the Core.

Grant Number: 5P20GM113123-10
NIH Institute/Center: NIH
Principal Investigator: David Bradley