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Establishing new model systems to study low potassium induced kidney injury
Organization UNIVERSITY OF PITTSBURGH AT PITTSBURGHLocation PITTSBURGH, UNITED STATESPosted 25 Jan 2024Deadline 31 Dec 2025 ⚠️
NIHUS FederalResearch GrantFY20253-D3-D modeling3-D structure3-Dimensional3-dimensional structure3D3D modeling3D structureAccelerationAgricultureAlternative Complement PathwayAmericanApicalBiologic ModelsBiological ModelsCardiovascular DiseasesCell BodyCell Culture TechniquesCell LineCell SurvivalCell ViabilityCell modelCell-Extracellular MatrixCellLineCellsCellular modelChronicChronic Kidney FailureChronic Renal DiseaseChronic Renal FailureCollagenComplement ActivationConsumptionCulturing, in vitro Vertebrate, PrimaryDevelopmentDidelphidaeDietDietary KDietary PotassiumDistal Convoluted TubuleDistal Kidney TubulesDistal convoluted renal tubule structureECMELISAElectron MicroscopyEndocrineEnvironmentEnzyme-Linked Immunosorbent AssayEpithelial CellsExtracellular MatrixFibrosisFoodFoundationsFundingGenesGlomerular Filtration RateGoalsGrantHealthHumanHypertensionHypokalemiaHypopotassemiaImmuneImmune Cell ActivationImmune responseImmunesImmunoblottingImmunofluorescenceImmunofluorescence ImmunologicImmunologic StimulationImmunological StimulationImmunostimulationIn VitroInflammationInjury to KidneyIntakeK elementKidneyKidney DiseasesKidney TubulesKidney Urinary SystemLinkMeasuresMediatingMethodologyMiceMice MammalsMicrofluidicsModel SystemModelingModern ManMorphogenesisMurineMusNIDDKNational Institute of Diabetes and Digestive and Kidney DiseasesNephropathyNutrientOpossumsPathologicPatientsPerfusionPermeabilityPhysiologyPilot ProjectsPlayPopulationPotassiumPotassium DeficiencyPredispositionPrimary Cell CulturesProcessProperdin PathwayProteinsProximal Kidney TubulesPublicationsRNA SeqRNA sequencingRNAseqRenal DiseaseRenal tubule structureResearchResearch MethodologyResearch MethodsRoleScientific PublicationSoilStrains Cell LinesStressStructureSurfaceSusceptibilitySystemTechnologyTestingTubularTubular formationVascular Hypertensive DiseaseVascular Hypertensive DisorderWestern BlottingWestern Immunoblottingaccess restrictionsbio-printingbioprintingcardiovascular disordercell culturecell cultureschronic kidney diseasecomplement pathway regulationcultured cell linecytokinedesigndesigningdevelopmentaldietsenzyme linked immunoassayextracellularfluid flowfruits and vegetablesglobal gene expressionglobal transcription profilehigh blood pressurehost responsehyperpiesiahyperpiesishypertensive diseasehypertensive disorderimmune activationimmune system responseimmunoresponseinflammation markerinflammatory markerinnovateinnovationinnovativekidney disorderkidney injurymechanical forcemonolayermorphogenetic processpilot studypressureprotein blottingrenalrenal disorderrenal injuryrenal proximal tubulerenal tubuleresearch and methodsresponsesalt hypertensionsalt induced hypertensionsalt sensitive hypertensionscaffoldscaffoldingshear stresssocial rolethree dimensionalthree dimensional structurethree-dimensional modelingtranscriptometranscriptome sequencingtranscriptomic sequencingwestern dietwestern-style dietwestern-type dietµfluidic
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ABSTRACT BOYD-SHIWARSKI
The purpose of this R03 proposal is to develop pilot studies using new research methodology and technology to
investigate how dietary potassium (K+) deficiency leads to kidney injury. It is well known that dietary K+ deficiency
leads to hypertension, cardiovascular disease, as well as chronic kidney disease. My NIDDK K08…
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