grant
Development, Validation, and Harmonization of Methods for Collection and Measurement of NAD+ and Related Metabolites: Application to a Randomized Trial of Patients Undergoing Coronary Artery Bypass
Organization BRIGHAM AND WOMEN'S HOSPITALLocation BOSTON, UNITED STATESPosted 1 Sept 2024Deadline 31 Aug 2029
NIHUS FederalResearch GrantFY202521+ years oldAcidsAcute Kidney FailureAcute Kidney InsufficiencyAcute Renal FailureAcute Renal InsufficiencyAddressAdultAdult HumanAgingAmericanAnimal ModelAnimal Models and Related StudiesAortaAortocoronary BypassAssayAttenuatedBioassayBiological AssayBiopsyBiopsy SampleBiopsy SpecimenCalibrationCardiacCell BodyCellsCenters for Disease ControlCenters for Disease Control and PreventionCenters for Disease Control and Prevention (U.S.)ClinicalClinical ResearchClinical StudyClinical TrialsCoenzyme IICollaborationsCollectionControlled Clinical TrialsCoronary Artery BypassCoronary Artery Bypass GraftingCoronary Artery Bypass SurgeryCoronary arterial bypassCustomDataDevelopmentDihydronicotinamide Adenine DinucleotideDiphosphopyridine NucleotideDiseaseDisorderDoseDouble-Blind MethodDouble-Blind StudyDouble-BlindedDouble-Masked MethodDouble-Masked StudyEndotheliumFatsFatty acid glycerol estersFunding OpportunitiesGlycohemoglobin AGlycosylated hemoglobin AHPLCHarvestHb A1Hb A1a+bHb A1cHbA1HbA1cHeart InjuriesHemoglobin A(1)High Performance Liquid ChromatographyHigh Pressure Liquid ChromatographyHigh Speed Liquid ChromatographyHumanHydrolaseHydrolase Family GeneHydrolase GeneInjuryIschemiaIschemia-Reperfusion InjuryKidneyKidney Urinary SystemLC/MSLaboratoriesLegLinkLiquid ChromatographyMeasurementMeasuresMethodologyMethodsModelingModern ManMuscleMuscle TissueMyocardialMyocardiumNAD phosphateNAD(H) phosphateNADHNADH phosphateNADPNADPHNadideNicotinamide MononucleotideNicotinamide adenine dinucleotideNicotinamide-Adenine DinucleotideNicotinamide-Adenine Dinucleotide PhosphateOperative ProceduresOperative Surgical ProceduresOralOral AdministrationOral Drug AdministrationOxidation-ReductionPARP PolymerasePARP proteinPARSPBMCPathologistPatient SchedulesPatientsPerchloric AcidsPeripheral Blood Mononuclear CellPhasePlacebo ControlPlacebosPlayPoly(ADP-ribose) PolymerasesPoly(ADPribose) PolymerasePostoperativePostoperative PeriodProceduresProcessProtocolProtocols documentationRandomization trialRandomizedRandomized, Controlled TrialsRedoxReference StandardsRegimenReperfusion DamageReperfusion InjuryResearch ProposalsResearch SpecimenRoleSamplingSham TreatmentSilent Mating Type Information Regulator 2-like ProteinsSir2-like ProteinsSirtuinsSkinSpecificitySpecimenStable Isotope LabelingStandardizationSurgicalSurgical InterventionsSurgical ProcedureTestingTherapeuticTissue HarvestingTrichloroacetic AcidTriphosphopyridine NucleotideUnited States Centers for Disease ControlUnited States Centers for Disease Control and PreventionValidationVariantVariationWhole Bloodacute kidney injuryadulthoodattenuateattenuatescardiac injurycardiac musclecofactorcollegecollegiatecoronary bypasscustomsdesigndesigningdevelopmentaldouble-blind placebo control trialdouble-blind placebo controlled trialdouble-masked controlled trialefficacy trialheart musclehemoglobin A1chuman studyhuman tissueinjuriesinjury to the myocardiuminterestintraoral drug deliveryliquid chromatography mass spectrometrymodel of animalmuscularmyocardial injuryoxidation reduction reactionplacebo controlledpoly ADP polymerasepoly ADP ribose synthetasepre-clinical studypreclinical studypreservationpreventpreventingpyridine nucleotiderandomisationrandomizationrandomized control trialrandomized trialrandomized, clinical trialsrandomly assignedrenalsample collectionsham therapysocial rolespecimen collectionsubcutaneoussubdermalsurgerytandem mass spectrometrytrichloro-acetic acidvalidations
Description preview
The methods used to measure NAD+ and NADH in human samples include the enzymatic cycling assay, high
pressure liquid chromatography and liquid chromatography tandem mass spectrometry (LC-MS/MS). The LC-
MS/MS method offers superior sensitivity, precision, accuracy compared to the other methods. However, all
current methods suffer from one or more…
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