Cytometry Facility

Abstract: Cytometry Facility (CF)
The Hillman Cancer Center (HCC) Cytometry Facility (CF) has been in continuous operation since 1991 and
has consistently provided HCC members with state-of-the-art cytometry instrumentation and expertise. Our
mission is to provide superior training, access to cutting edge instrumentation, expertise in assay
development and data analysis, and opportunities for cross-collaboration with other shared
resources at HCC. CF has been located in the HCC Research Pavilion since September 2002. The
laboratory consists of a 1000 ft2 laboratory dedicated to preparative and analytical flow cytometry. CF
provides services to over 100 users within HCC. The current instrumentation includes a Beckman Coulter
MoFlo Astrios high-speed cell sorter equipped with a Class II Biocontainment hood, two Becton Dickinson
15/18-color SORP Fortessa analyzers with custom high-power lasers, and a Becton Dickinson Accuri C6 4-
color analyzer. Recent additions of a Sony MA900 cell sorter, also equipped with a Class II Biocontainment
hood, and a Cytek Aurora, a spectral cytometer, have addressed the increasing demand for more access to
state-of-the-art instrumentation. Education initiatives have expanded considerably, including a tiered system
of user expertise, advanced education seminars on spectral cytometry, and user-operated cell sorting on the
Sony MA900. The Specific Aims focus on providing access and training on existing equipment, integrating
new equipment, and providing expert research staff and continuing education to users on new experimental
methods.

Grant Number: 4P30CA047904-37
NIH Institute/Center: NIH
Principal Investigator: Tullia Bruno