grant

Antibody Mediated Immunity Against Brucella

Organization UNIVERSITY OF MISSOURI-COLUMBIALocation COLUMBIA, UNITED STATESPosted 25 Jul 2024Deadline 30 Jun 2027
NIHUS FederalResearch GrantFY202519S Gamma GlobulinAb responseAb-mediated immunityAb-mediated protectionAffectAnimalsAntibodiesAntibody FormationAntibody ProductionAntibody immunityAntibody protectionAntibody-mediated protectionAttenuated VaccinesB blood cellsB cellB cellsB melitensisB-CellsB-LymphocytesB-cellB. melitensisBody TissuesBrucellaBrucella VaccineBrucella melitensisBrucellosisCell BodyCellsClass SwitchingClass SwitchingsComplementComplement ProteinsComplement ReceptorD-GlucoseDNA mutationDataDendritic CellsDextroseDiseaseDisorderExposure toFarm AnimalFc ReceptorFutureGC MSGCMSGas-Liquid-Mass Spectrometry ChromatographyGenesGenetic ChangeGenetic defectGenetic mutationGenomeGlucoseGlucose Binding ProteinGlucose Transport ProteinGlucose TransporterGoalsHumanHumoral ImmunitiesIgMImmunityImmunoglobulin Class SwitchingImmunoglobulin Class SwitchingsImmunoglobulin MInfectionInfection ControlIntermediary MetabolismIsotype SwitchingIsotype SwitchingsKnowledgeLicensingLive-attenuated VaccineLivestockLong-term infectionMacrophageMalta FeverMass FragmentographiesMass FragmentographyMass-Gas Chromatography SpectrometryMass-Gas Chromatography Spectrum AnalysisMediatingMetabolic ProcessesMetabolismMiceMice MammalsModern ManMurineMusMutationOutputPathogenicity FactorsPatientsPredispositionPrimary InfectionProteinsRoleSiteSurfaceSusceptibilityTestingTissuesUndulant FeverVaccinatedVaccinationVaccinesVeiled CellsVirulenceVirulence FactorsWild Type MouseZoonosesZoonoticZoonotic Infectionadaptive immunityantibody biosynthesisantibody receptorantibody-based immunityantibody-mediated immunitychronic infectioncomplementationextracellulargenome mutationimmunoglobulin biosynthesisimprovedin vivoion trap mass spectrometrylive vaccinelive vaccinesmass fragmentometrymetabolic profilemutantneglectpathogenpersistent infectionpreventpreventingrational designsocial rolewildtype mouse
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Full Description

Abstract:
No vaccines are currently licensed to prevent human brucellosis and existing vaccines for

livestock are not entirely efficacious. In humans and other animals, Brucella can cause a lifelong

infection. However, mechanisms underlying the ability of Brucella to subvert adaptive immunity

remain unclear. In this proposal we show that vaccine elicited antibodies alter host metabolism to

protect the host against Brucella to some degree. Therefore, in Specific Aim #1 of this proposal

we will determine how vaccine-elicited IgM and class switched antibodies alter tissue metabolism

to restrict Brucella infection. We also found that Brucella encodes virulence factors that mediate

evasion of humoral immunity. Therefore, in Specific Aim #2 of this proposal we will identify

mechanisms by which Brucella subverts antibody mediated immunity. Collectively, our results will

enhance our understanding of how current vaccines protect the host, and how Brucella is able to

subvert adaptive immunity, and thus could improve the rational design of highly effective vaccines

for Brucella.

Grant Number: 5R21AI185488-02
NIH Institute/Center: NIH

Principal Investigator: Mostafa Ateya Abushahba

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Antibody Mediated Immunity Against Brucella — UNIVERSITY OF MISSOURI-COLUMBIA | UNITED STATES | Jul 2024 | Dev Procure