grant
Zebrafish targeted mutagenesis and functional genomics
Organization NATIONAL HUMAN GENOME RESEARCH INSTITUTELocation UNITED STATES
NIHUS FederalResearch GrantFY2025Blood capillariesBrachydanio rerioCRISPRCRISPR approachCRISPR based approachCRISPR methodCRISPR methodologyCRISPR techniqueCRISPR technologyCRISPR toolsCRISPR-CAS-9CRISPR-based methodCRISPR-based techniqueCRISPR-based technologyCRISPR-based toolCRISPR/CAS approachCRISPR/Cas methodCRISPR/Cas systemCRISPR/Cas technologyCRISPR/Cas9CRISPR/Cas9 technologyCas nuclease technologyClustered Regularly Interspaced Short Palindromic RepeatsClustered Regularly Interspaced Short Palindromic Repeats approachClustered Regularly Interspaced Short Palindromic Repeats methodClustered Regularly Interspaced Short Palindromic Repeats methodologyClustered Regularly Interspaced Short Palindromic Repeats techniqueClustered Regularly Interspaced Short Palindromic Repeats technologyCommunitiesComputer softwareDNA Damage RepairDNA RepairDNA mutationDanio rerioDataDefectDevelopmental GeneDropsEnhancersGene TargetingGenesGenetic ChangeGenetic DiseasesGenetic ScreeningGenetic defectGenetic mutationGenetics-MutagenesisGenomeGerm LinesGoalsGuide RNAHuman GeneticsKnock-outKnockoutLengthModelingMutagenesisMutagenesis Molecular BiologyMutationProcessPublicationsResearchResearch ResourcesResourcesRestScientific PublicationSoftwareTechniquesTechnologyTestingTransgenesTransmissionUnscheduled DNA SynthesisWritingZebra DanioZebra FishZebrafishbasebase editorbasescapillarycost effectivefunctional genomicsgRNAgene functiongene locusgene regulatory networkgenetic conditiongenetic disordergenetic locusgenome mutationgenome scalegenome-widegenomewidegenomic locationgenomic locushigh throughput technologyhuman diseasehuman modelimprovedmodel of humanpan-genomepangenomeprime editingprime editorprogramssite-specific integrationtooltransgenetransmission processzebrafish genome
Description preview
The primary focus of the lab is to develop tools for studying gene function on a genome wide scale.
We use the bacterial CRISPR/Cas9 gene-targeting technique. This provides us with a cost effective way to efficiently target gene KO's in zebrafish. We have developed a streamlined pipeline for gene targeting using CRISPR/Cas9 where we use…
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