grant
Single-molecule analysis of eukaryotic transcription activation
Organization HARVARD MEDICAL SCHOOLLocation BOSTON, UNITED STATESPosted 7 Feb 2020Deadline 30 Jun 2028
NIHUS FederalResearch GrantFY2025Active Follow-upAddressAffectBaker's YeastBasal Transcription FactorBasal transcription factor genesBehaviorBindingBinding SitesBiochemicalBiologic ModelsBiological ModelsBrewer's YeastCancer GenesCancer-Promoting GeneCancersCell BodyCellsCharacteristicsChromatinColorCombining SiteComplementComplement ProteinsComplexDNADNA Binding DomainDNA-Binding Protein MotifsDNA-Dependent RNA Polymerase IIDeoxyribonucleic AcidDiseaseDisorderDrugsEngineeringEnhancersEukaryotaEukaryoteEventFaceFluorescenceFrequenciesGene Action RegulationGene ExpressionGene Expression RegulationGene RegulationGene Regulation ProcessGene TranscriptionGeneHomologGeneral Transcription Factor GeneGeneral Transcription FactorsGenetic TranscriptionGenomicsGleanGoalsHomologHomologous GeneHomologueImageImmobilizationKinasesLabelMalignant CellMalignant NeoplasmsMalignant TumorMeasurableMeasurementMeasuresMediatorMedicationMethodsMicroscopeMicroscopyModel SystemModelingModificationMolecularMolecular InteractionMonitorMutateNuclearNuclear ExtractNucleosomesOncogenesPharmaceutical PreparationsPhosphotransferase GenePhosphotransferasesPre-mRNAProcessPropertyRNA ExpressionRNA Polymerase BRNA Polymerase IIRNA SplicingRNA, Messenger, PrecursorsReactive SiteRoleS cerevisiaeS. cerevisiaeSAGASPT/ADA/Gcn5 AcetyltransferaseSaccharomyces cerevisiaeSideSlideSpectroscopySpectrum AnalysesSpectrum AnalysisSplicingSystemTIRF MicroscopyTailTechniquesTechnologyTestingTotal Internal Reflection FluorescentTotal Internal Reflection Fluorescent MicroscopyTranscriptionTranscription ActivationTranscription ActivatorTranscription CoactivatorTranscription Factor CoactivatorTranscription Factor Proto-OncogeneTranscription InitiationTranscription factor genesTranscriptional ActivationTranscriptional Activator/CoactivatorTransfer RNATransforming GenesTransphosphorylasesTriplet Codon-Amino Acid AdaptorVP 16VP16WorkYeastsactive followupcancer cellcomplementationdrug/agentexperienceexperimentexperimental researchexperimental studyexperimentsfacesfacialfollow upfollow-upfollowed upfollowuphistone modificationimagingintervention designlight microscopymRNA Precursormalignancymutantneoplasm/cancerorthopedic freezingpromoterpromotorrecruitresidenceresidential buildingresidential siteresponsesingle moleculesocial rolesynergismtRNAtherapy designtranscription factortransfer Ribonucleic acidstreatment design
Description preview
Project Summary/Abstract. The goal of this project is to better understand activation of transcription initiation
by eukaryotic RNA polymerase II (RNApII), a process that is often abnormal in cancer cells. Our system
combines Colocalization Single-Molecule Spectroscopy (CoSMoS, a TIRF microscopy technique for
simultaneously analyzing hundreds of…
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