NSF-DBT: Structural and Biophysical Analysis of Evolved Fe(II)/2-Ketoglutarate-Dependent Azidases to Understand and Expand the Substrate Scope of Enzymatic C-H Functionalization

Enzymes promote reactions by binding the substrate, catalyzing the reaction, and releasing the product. Each of these activities depends on the three-dimensional shape of the enzyme. Efforts to enhance the overall productivity of an enzyme usually focus on increasing the rate of the reaction in the active site. However, altering the shape to…