grant

Nitrogen Metabolism of Staphylococcus aureus

Organization UNIVERSITY OF NEBRASKA MEDICAL CENTERLocation OMAHA, UNITED STATESPosted 2 Feb 2025Deadline 1 Feb 2030
NIHUS FederalResearch GrantFY2026AcidsAddressAerobicAffectAmino AcidsAmmoniumAnabolismAnimalsArg-GluArginineB subtilisB. subtilisBacillus subtilisBacteremiaBacteriaBand Shift Mobility AssayBandshift Mobility AssayBiologyBloodBlood Reticuloendothelial SystemCarbamideCarbonCatabolismChemicalsClinical geneticsCollaborationsCommunicable DiseasesComplexD-GlucoseDNA BindingDNA Binding InteractionDNA boundDataDefectDependenceDextroseDiseaseDisorderElaqua XXElectrophoretic Mobility Shift AssayEndocarditisEnsureEnvironmentEnzyme GeneEnzymesFacultyFeedbackFosteringGene TranscriptionGeneralized GrowthGenesGeneticGenetic TranscriptionGenus staphylococcusGlnGlucoseGlutamate Ammonia Ligase (ADP)Glutamate-Ammonia LigaseGlutamatesGlutamineGlutamine SynthetaseGram-Positive BacteriaGrowthHistidineHumanIndividualInfectionInfectious DiseasesInfectious DisorderInfectious Skin DiseasesInfective endocarditisIntermediary MetabolismIonsKnowledgeL-ArginineL-GlutamateL-GlutamineL-ProlineL-SerineLabelLaboratoriesMedical GeneticsMedical centerMentorsMentorshipMetabolicMetabolic ProcessesMetabolismMiceMice MammalsMobility Shift AssayModern ManMurineMusNO3-NatureNebraskaNitrate ReductasesNitratesNitritesNitrogenNitrogen FixationNitrogen Fixation GenesNitrogen Metabolism PathwayNitrogen Regulator GenesNitrogen RegulatorsNucleotide BiosynthesisNutrientOligopeptidesPathogenesisPathway interactionsPeptide TransportPhenotypePhysiciansPhysiologicPhysiologicalPopulationProcessProlineQ LevoglutamideQ. LevoglutamideQuantitative RTPCRQuantitative Reverse Transcriptase PCRRNA ExpressionRNA SeqRNA sequencingRNAseqReactionRegulationReportingResearchRoleRouteS aureusS. aureusS. aureus infectionScientistSenior ScientistSerineSkinSkin TissueSoft Tissue InfectionsSourceStaph aureusStaph aureus infectionStaphylococcusStaphylococcus aureusStaphylococcus aureus infectionSystems BiologyTissue GrowthTissue ModelTrainingTranscriptionUniversitiesUreaUrea CarbamideUreaphilVirulenceaminoacidarginine glutamatearginylglutamatebacteraemiabacteria metabolismbacterial bloodstream infectionbacterial geneticsbacterial infection in the bloodstreambacterial metabolismbiosynthesiscareercutaneous infectioncutaneous tissueexperimentexperimental researchexperimental studyexperimentsgel shift assayglutamate synthetaseglutamatergicglutamine synthaseinfected skininfected with S. aureusinfected with Staph aureusinfected with Staphylococcus aureusmeetingmeetingsmembermetabolism measurementmetabolomicsmetabonomicsmetermicrobiomemouse modelmurine modelmutantnew therapeutic approachnew therapeutic interventionnew therapeutic strategiesnew therapy approachesnew treatment approachnew treatment strategynitratenitrogen metabolismnovel therapeutic approachnovel therapeutic interventionnovel therapeutic strategiesnovel therapy approachontogenypathogenpathwaypermeasepromoterpromotorqRTPCRquinol-nitrate oxidoreductaserespiratory nitrate reductaseresponseskin infectionsocial rolesoft tissuetranscriptome sequencingtranscriptomic sequencingvitamin biosynthesis
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PROJECT SUMMARY
Staphylococcus aureus is a gram-positive bacterium that colonizes the nares of ~30% of the human population

and is the most common cause of bacteremia, endocarditis, and skin and soft tissue infections. Environmental

nitrogen acquisition is essential for bacterial growth. To meet intracellular nitrogen requirements, S. aureus…

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Nitrogen Metabolism of Staphylococcus aureus — UNIVERSITY OF NEBRASKA MEDICAL CENTER | UNITED STATES | Feb 2025 | Dev Procure