grant
NHGRI/DIR Embryonic Stem Cell and Transgenic Mouse Core
Organization NATIONAL HUMAN GENOME RESEARCH INSTITUTELocation UNITED STATES
NIHUS FederalResearch GrantFY2025AllelesAllelomorphsAnimalsArchivesAreaBacteriaBreedingCRISPRCRISPR/Cas systemCell BodyCell LineCellLineCellsChimeraChimera organismClustered Regularly Interspaced Short Palindromic RepeatsCommunitiesCryofixationCryopreservationDNADeoxyribonucleic AcidDisastersDissectionDysembryomaES Cell LineES cellElectroporationEmbryoEmbryonicEmbryonic Stem Cell LineExtracellular Signal-Regulated Kinase GeneFacial VeinFacial vein structureFemaleFertilization in VitroFertilized EggFertilized OvumFibroblastsFreezingGEM modelGEMM modelGene ModifiedGene TargetingGene Transfer TechniquesGenerationsGenesGenetic DiseasesGenetically Engineered MouseGenotypeGoalsGuide RNAHarvestHuman GeneticsHybrid CellsHybridsIn VitroInjectionsInnate ImmunityInvestigatorsKO miceKnock-out MiceKnockout MiceLocationMAP Kinase GeneMAPKMethodsMiceMice MammalsMitogen-Activated Protein Kinase GeneModelingMurineMusMutant Strains MiceNHGRINational Center for Human Genome ResearchNational Human Genome Research InstituteNative ImmunityNatural ImmunityNon-Specific ImmunityNonspecific ImmunityNull MousePathway interactionsProceduresProductionProtocolProtocols documentationReagentResearchResearch PersonnelResearch SupportResearchersServicesSiteSomatic Cell HybridsSpermSpermatozoaStandardizationStrains Cell LinesSystemTechniquesTechnologyTeratoid TumorTeratomaTest-Tube FertilizationTimeTransfectionTransgenesisTransgenic MiceTransgenic OrganismsWorkchimerascold preservationcold storagecultured cell linedesign and constructdesign and constructionelectroporative deliveryembryo cellembryo derived stem cellembryonal stem cellsembryonic progenitorembryonic stem cellexperimentexperimental researchexperimental studyexperimentsgRNAgene electrotransfergene modificationgenetic conditiongenetic disordergenetically engineered mouse modelgenetically engineered murine modelgenetically modifiedgenome editinggenomic editingiPSiPSCiPSCsimmunocytochemistryin uteroin vivoinduced pluripotent cellinduced pluripotent stem cellinducible pluripotent cellinducible pluripotent stem cellinhibitormalemodel organismmouse modelmouse mutantmurine modelmutant mouse modelnucleasepathwaypluripotencypluripotent stateprogramssperm cellstem cell of embryonic originstem cell technologytransgenicuser-friendlyzoospermzygote♀♂
Description preview
The NHGRI Embryonic Stem Cell and Transgenic Mouse Core provides a shared service to NHGRI investigators. The Core specializes in generating genetically engineered mice (GEM) via conventional transgenesis, ES cell targeting, but primarily through genome editing using site-specific nucleases (CRISPR/Cas). To do this work, the Core breeds, in-house,…
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