grant
Immunophenotyping by high-dimensional cytometry and machine learning
Organization DIVISION OF BASIC SCIENCES - NCILocation UNITED STATES
NIHUS FederalResearch GrantFY2025AntibodiesAntigensAutoregulationBiologicalBlood leukocyteBlood monocyteBone MarrowBone Marrow Reticuloendothelial SystemCell BodyCell DifferentiationCell Differentiation processCell Growth in NumberCell MultiplicationCell ProliferationCellsCellular ProliferationClinical ResearchClinical StudyCoinCollaborationsCommunicationCytokine Signal TransductionCytokine SignalingCytometryDrugsEquilibriumFlow CytofluorometriesFlow CytofluorometryFlow CytometryFlow MicrofluorimetryFlow MicrofluorometryGoalsGrantHomeostasisImmunologic SubtypingImmunomodulationImmunophenotypingInflammationLeukocytesLeukocytes Reticuloendothelial SystemMachine LearningMarrow leukocyteMarrow monocyteMeasurementMeasuresMedicationMethodologyMethodsMiceMice MammalsModelingMurineMusNational Institutes of HealthPharmaceutical PreparationsPhenotypePhysiologic pulsePhysiological HomeostasisPopulationPulsePythonsR-Series Research ProjectsR01 MechanismR01 ProgramRegulationResearchResearch GrantsResearch Project GrantsResearch ProjectsResolutionSeriesSignal PathwaySiteT-CellsT-LymphocyteTechniquesUnited States National Institutes of HealthWhite Blood CellsWhite CellWorkbalancebalance functionbio-informatics pipelinebioinformatics pipelinebiologiccellular differentiationcytokinedrug/agentflow cytophotometryhigh dimensionalityimmune modulationimmune regulationimmunogenimmunologic reactivity controlimmunomodulatoryimmunoregulationimmunoregulatorymachine based learningmonocytepre-clinical studypreclinical studyresolutionsresponsethymus derived lymphocytetumorwhite blood cellwhite blood corpuscle
Description preview
We recently transitioned to spectral flow cytometry (instead of mass cytometry): this is faster, cheaper, more reliable, high-througput experimental techniques with no downside in terms of the measurement quality. We have also introduced a series of Python pipeline to streamline the processing and interpretation of large corpus of experimental…
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