A novel genetic switch with an optimal ON/OFF ratio to preserve growth performance prior to Escherichia coli autolysis for enhanced plasmid release

When the biotechnology workhorse organism, Escherichia coli (E. coli) is used to propagate commercially valuable plasmids, such as those demanded by the globally expanding cell and gene therapy sector, efficient recovery of plasmids from the E. coli cell is key. However, this recovery of the desired plasmid product can prove challenging, with…